AID ImmunoArray Method – Reverse hybridization of DNA

AID DNA Arrays are based on reverse hybridization of DNA.

Reverse hybridization enables:

• Detection of SNPs (single nucleotide polymorphism)
• Differentiation between homozygous and heterozygous genotype
• Characterization of alleles in polymorphic regions like HLA gen complex
• Detection of viral and bacterial DNA

DNA amplification by polymerase chain reaction (PCR) before DNA characterization enables high sensitivity. Minimal amounts of DNA are sufficient for analysis.

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Characteristics

Advantages of AID Array at a glance:

• Highly reliable, reproducible and simple method
• Little time needed: reverse hybridization lasts 2.5 hours
• No dangerous ethidiumbromid gel electrophoresis necessary
• Included positive- and negative controls document a valid result
• 96 well microtiter plate format suited for moderate and high sample throughput
• Automated workstation available
• Evaluation and documentation by AID Reader and AIDot software
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Reader-Systems

AIDot