Detection of Shared Epitope HLA-DRB1 alleles including subtypes DR1 and DR4
Rheumatoid arthritis (RA) or chronic polyarthritis is an intermittent systemic autoimmune disease which occurs in approx. 1% of the population. The etiology of the disease is unknown.
There are great differences in progression of the disease in different patients. Therefor a early diagnosis is essential for optimal therapy to avoid long-term damages.
It has been shown by studies that there is a genetic disposition for RA caused by several alleles of the HLA-DR region. RA is associated with the HLA- DRB1 alleles coding for amino acid motives QKRAA (*0401), QRRAA (*0404, *0405, *0408, *0101, *0102, *1402) or RRRAA (*1001), named „rheumatoid epitope" or „Shared Epitope“. About 80-90% of all Caucasian RA patients are heterozygous or homozygous for one of these HLA-DR-Shared Epitope alleles.
AID Shared Epitope Kit enables the detection of HLA-risk alleles for RA.
- Detection of Shared Epitope Genotype by screening of HLA-DRB1 allele and subtyping of DR1 and DR4
- Differentiation between homozygous and heterozygous genotype
- PCR with subsequent lineprobe assay
- Specimen: human DNA isolated from buccal swab, Citrate- or EDTA-blood or biopsy
- Control bands on every strip show correct DNA isolation, amplification and hybridization
- Results within 4 hours
- Suited for automated systems
- Evaluation and documentation with AID Scanning system
- Art.no.: RDB 2035