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SARS-CoV-2 PCR

SARS-CoV-2 Mutation Screening

The AID SARS-CoV-2 MutaScreen21 PCR kit is a diagnostic PCR kit for the qualitative detection and differentiation of characteristic Mutations in the SARS-CoV-2 spike gene.

It differentiates the mutations 69-70del, 417N/T, 439K, 452R, 478K, 484A/K/Q and 501Y.

The combination of those mutations characterises different variants of SARS-CoV-2, including the variants of concern Alpha, Beta, Gamma, Delta and Omicron.

Variant/Mutation

69-70
del

417
N/T
439K 452R 478K 484
A/K/Q
501Y
B.1.1.7 Alpha           (K)
B.1.351 Beta     (N)        K
B.1.1.28.1 Gamma     T        K
B.1.617.2 Delta           
B.1.617.1 Kappa           Q  
B.1.617.3           Q  
B.1.525 Eta           K  
B.1.147/B.1.429
Epsilon 
           
B.1.526 Iota             (K)  
B.1.526.1             
B.1.1.28.2 Zeta             K  
B.1.258           
B.1.1529.BA.1
Omicron 
 N    A
B.1.1529.BA.2
Omicron 
   N    A

(): Not found in all isolates

Characteristics

  • PCR with subsequent lineprobe assay
  • Detection of mutations or deletions 69-70del, 417N/T, 439K, 452R, 478K, 484A/K/Q and 501Y of SARS-CoV-2 and thereby characterised variants of concern
  • Specimen: RNA from nasal or pharyngeal swab
  • Results within 4 hours
  • Suited for automated systems
  • Control bands on every strip show correct RNA isolation, amplification and hybridization
  • Evaluation and documentation with AID Scanning system
  • Art.no.: RDB 2340
  •