Molecular Genetic Assay Method – Reverse hybridization

AID molecular genetic diagnostic kits are based on reverse hybridization of DNA.

Reverse hybridization enables:

• Detection of SNPs (single nucleotide polymorphism)
• Differentiation between homozygous and heterozygous genotype
• Characterization of alleles in polymorphic regions like HLA gen complex
• Detection of viral and bacterial DNA

DNA amplification by polymerase chain reaction (PCR) before DNA characterization enables high sensitivity. Minimal amounts of DNA are sufficient for analysis.

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Advantages of AID molecular genetic diagnostic kits at a glance:

• Highly reliable, reproducible and simple method
• Little time needed: reverse hybridization lasts 2.5 hours
• Rational workflow: same procedure and reagents for all kits enables parallel implementing of several parameters
• Little lab cost by little equipment needed: Thermocycler and shaking water bath
• No dangerous ethidiumbromid gel electrophoresis necessary
• Included positive- and negative controls document a valid result
• Quick and easy evaluation of the strips by AID Immunblot/Genoblot-Scanning system
• Suited for small, moderate and high sample throughput
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Strips can be evaluated by AID Immunblot/Genoblot-Scanning System quick and easily.