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PCR detection of ESBL genes

AID ESBL PCR kit – detection of major mutations in TEM, SHV, CTX-M and KPC

Fast and reliable detection of ESBL genes

Increasing prevalence of resistance against cephalosporins in extended-spectrum ß-lactamase (ESBL)-producing Enterobacteriaceae has continuously been reported over the past years.

Depending on the ESBL type, these b-lactamases have the ability to hydrolyse various penicillins, first-, second- and third-generation cephalosporins, and aztreonam (but not cephamycins or carbapenems). Currently, the most prevalent ESBLs include TEM, SHV and CTX-M enzyme types.

Phenotypic susceptibility testing for ESBLs may be complicated by the presence of multiple ß-lactamases in a single bacterial strain, e.g. ESBLs, AmpCs and carbapenemases, and by different levels of b-lactamase gene expression.Genotypic tests have the potential to accurately identify different ß-lactamase genes
and mutations that result in ESBL.

Characteristics

  • Developed for rapid detection of ESBL-genes within 5 hours
  • Detection of the most important mutations in TEM and SHV as well as CTX-M and the Klebsiella pneumoniae Carbapenemase (KPC)
  • For culture and clinical specimens
  • Suited for automated systems
  • Control bands on every strip shows correct DNA isolation, amplification and hybridization
  • Evaluation and documentation with AID scanner and software
  • Art.no.: RDB 2180

 

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